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GE/Pharmacia systems utilize two two-cylinder piston pumps, one for each buffer, combining the output of both in a mixing chamber. Waters systems use a single peristaltic pump which draws both buffers from separate reservoirs through a proportioning valve and mixing chamber. Fast protein liquid chromatography (FPLC) is a form of medium-pressure chromatography that uses a pump to control the speed at which the mobile phase passes through the stationary phase. FPLC was introduced in 1982 by Pharmacia as fast performance liquid chromatography. FPLC Systems FPLC stands for fast protein liquid chromatography. Fast protein liquid chromatography (FPLC) is a form of high-performance liquid chromatography (HPLC). FPLC utilizes a small diameter stationary phase which enables higher resolution than achievable with. Fplc column. Pharmacia LKB FPLC System with 2 P500, LCC-501 Plus Ctrl, FRAC-100, P-1, Conductivity Monitor, UV-M Download High Res Photos Make an Inquiry Add To Watch List Finance for as low as. $99.00 / month Click to Apply Now! Pharmacia FPLC System This column chromatography system automates the separation and purification of semi-preparative amounts of the compound of interest, typically a protein.